Laboratory for human molecular genetics – Projects

Laboratory for human molecular genetics – Projects

Studying signal transduction pathways and epigenetic mechanisms that control human SOX genes expression: further insight into their roles in cell fate determination and differentiation (funded by MSTDS, 2011-2014).

Ethnogenesis of the Serbs during the Middle Ages (until c.1250): a comparative analysis of the historic-cultural heritage, genetic material and artifacts of material culture from the aspect of analytical chemistry (funded by MSTDS, 2011-2014).

Regulation of human SOX18 gene expression and its role in angiogenesis (funded by ICGEB, 2008-2010).

Studying the regulation of expression and function of the human SOX genes (funded by MSRS, 2006-2010).

Human SOX genes: Studying the structure and regulation of expression (funded by MSEPRS, 2002-2005).

Retinoic acid induction of the SOX3 gene expression (funded by ICGEB, 2001-2003).

Development and application of novel biotechnological methods and procedures for enlargement of production and improvement of the quality of meat, milk and eggs (funded by MSTRS, 1998-2000).

FISH (fluorescent in situ hybridization) and its application in prenatal diagnosis, detection of inherited diseases and in malignant transformation (funded by MSTRS, 1997-1998).

Cloning and characterization of human SOX genes (funded by MSTRS, 1996-2000).

SOX3 activation by retinoic acid (The Wellcome Trust, UK, 18876GCS-B09, 1995-1997).

SOX genes: A new family of genes related to the sex determining gene SRY (The Wellcome Trust, UK, 037356/Z/92, 1992-1993).

Research interests :

1. Studying the regulation of expression and function of the human SOX genes

Current specific research goals:

      - Studying the structure and transcriptional regulation of human SOX genes expression.

      - Identification of transcription factors and signaling pathways involved in the regulation of the human SOX genes expression.

      - Manipulation of SOX2 and SOX3 expression during neural differentiation of NT2/D1 cells that resemble early embryonic stem cells.

      - Studying the roles of SOX genes in neural differentiation and angiogenesis.

The ongoing research will enable identification of signaling networks that are involved in the regulation of SOX genes expression and will contribute to the better understanding of the roles of these genes in neural differentiation and angiogenesis.

2. Ethnogenesis of the Serbs during the Middle Ages

Specific research goals:

      - High-resolution phylogenetic studies of mtDNA and Y chromosome variation in ancient populations thatresided in the Southwestern region of Serbia from Bronze Age up to Middle Ages (1800 BC-1250 AD).

      - The geographic distribution and diversity of genetic variation.

      - Comparison of the variability between ancient and contemporary populations that will provide an overviewof the migration patterns which led to the colonization of the various geographic areas of Serbia.

3. Application of Fluorescent in situ Hybridization (FISH) for detection of numerical and structural chromosome aberrations

FISH based diagnostics was developed for prenatal and postnatal detection of chromosome aberrations in humans for:

      - Diagnostics of micro-deletion syndromes (DiGeorge and Prader-Willi / Angelman).

      - Detection of rare mosaic karyotypes.

      - Detection of the complex structural chromosomal rearrangements.

4. Marker assisted selection for the economic trait loci in cattle

      - PCR and subsequent restriction fragment length polymorphism analysis (PCR-RFLP) have been used for genotyping milk protein variants in cattle. Accurate genotyping of k-casein and b-lactoglobulin and selection of desirable genotypes have economic significance since their genetic variants have effects on milk production traits .

      - We have also developed molecular diagnostics of BLAD ( Bovine Leukocyte Adhesion Deficiency) and porcine stress syndrome.

updated 02.02.2012